C1Q ASSAY RESULTS IN COMPLEMENT-DEPENDENT CYTOTOXICITY CROSSMATCH NEGATIVE RENAL TRANSPLANT CANDIDATES WITH DONOR-SPECIFIC ANTIBODIES: HIGH SPECIFICITY BUT LOW SENSITIVITY WHEN PREDICTING FLOW CROSSMATCH

CH 28 : José M. Arreola-Guerra, Luis E. Morales-Buenrostro, Natalia Castelán, Adrián de Santiago, Adriana Arvizu, Norma Gonzalez-Tableros, Mayra López, Mario Vilatobá, Julio Granados, and Josefina Alberú

Abstract

In complement dependent cytotoxicity crossmatch negative renal transplant candidates with human leukocyte antigen donor-specific antibodies (DSA), both the presence of DSA C1q+ and the dominant DSA fluorescence were significantly associated with a positive flow cytometry crossmatch (FXM+). The C1q+ assay was highly specific, but had low sensitivity when predicting FXM+, so the clinical significance of a FXM+ in the absence

of DSA C1q+ remains to be clarified in future studies.