Events

14
Oct
2020

Terasaki Talks Presents: "Protein Engineering of Multi-functional Biomaterials for Regenerative Medicine", Presenter: Dr. Sarah Heilshorn

10-14-2020 8:00 am -9:00 am
 Terasaki Talks Presents: "Protein Engineering of Multi-functional Biomaterials for Regenerative Medicine", Presenter: Dr. Sarah Heilshorn

Abstract:

Stem cell transplantation is a promising therapy for a myriad of debilitating diseases and injuries; however, current expansion and transplantation protocols are inadequate. My lab designs biomaterials to overcome these challenges using biomimetic, protein-engineering technology. By integrating protein science methodologies with simple polymer physics models, we manipulate the polypeptide chain interactions and demonstrate the direct ability to tune the material properties including hydrogel mechanics, cell-adhesion, and biodegradation. These materials have allowed us to identify matrix remodeling as a previously unknown requirement for maintenance of stemness in neural progenitor cells within 3D expansion systems. Through a series of in vitro and in vivo studies, we demonstrate that protein-engineered hydrogels may significantly improve transplanted stem cell retention and regenerative function. Furthermore, many of the lessons learned about designing injectable biomaterials can be extended to design new bio-inks for 3D printing applications. While 3D printing has enormous potential for tissue engineering, few bio-inks are currently available to facilitate the printing of complex, cell-laden constructs. We demonstrate the design of customizable bio-inks that enable the printing of multiple cell types into distinct geometric forms.

28
Oct
2020

Terasaki Talks Presents: “Additively Manufactured Porous Titanium Scaffolds: Cell Proliferation”, Presenter: Dr. Bingbing Li

10-28-2020 8:00 am -9:00 am
Terasaki Talks Presents: “Additively Manufactured Porous Titanium Scaffolds: Cell Proliferation”, Presenter: Dr. Bingbing Li

Abstract:

Pore size, external shape, and internal complexity of additively manufactured porous titanium scaffolds are three primary determinants of cell viability and structural strength of scaffolds in bone tissue engineering. To obtain an optimal design with the combination of all three determinants, four scaffolds each with a unique topology (external geometry and internal structure) were designed and varied the pore sizes of each scaffold 3 times. For each topology, scaffolds with pore sizes of 300, 400, and 500 µm were designed. All designed scaffolds were additively manufactured in material Ti6Al4V by the direct metal laser melting machine. Compression test was conducted on the scaffolds to assure meeting minimum compressive strength of human bone. The effects of pore size and topology on the cell viability of the scaffolds were analyzed. The 12 scaffolds were ultrasonically cleaned and seeded with NIH3T3 cells. Each scaffold was seeded with 1 million cells. After 32 days of culturing, the cells were fixed for their three-dimensional architecture preservation and to obtain scanning electron microscope images.

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